https://novaprd-lb.newcastle.edu.au/vital/access/manager/Index ${session.getAttribute("locale")} 5 Necroptosis signaling promotes inflammation, airway remodeling, and emphysema in chronic obstructive pulmonary disease https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:49965 Wed 21 Jun 2023 11:57:33 AEST ]]> Causes and consequences of oxidative stress in spermatozoa https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:24338 Wed 17 Nov 2021 16:29:37 AEDT ]]> Histone deacetylases (HDACs) as mediators of resistance to apoptosis in melanoma and as targets for combination therapy with selective BRAF inhibitors https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:27649 Wed 17 May 2017 11:47:32 AEST ]]> Functional relevance of SATB1 in immune regulation and tumorigenesis https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:33086 Wed 15 Dec 2021 16:08:07 AEDT ]]> Chemosensitization of solid tumors by inhibition of Bcl-xL expression using DNAzyme https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:20299 Wed 11 Apr 2018 16:54:44 AEST ]]> MEK-independent survival of B-RAFV600E melanoma cells selected for resistance to apoptosis induced by the RAF inhibitor PLX4720 https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15166 V600E melanoma cells to B-RAF inhibitors. Experimental Design: B-RAF^V600E melanoma cells were exposed to the B-RAF inhibitor PLX4720 for prolonged periods to select for cells resistant to apoptosis induced by the inhibitor. The resultant cells were analyzed for activation of extracellular signal regulated kinase (ERK), MAP/ERK kinase (MEK), and Akt, and related signals. Their roles in survival of the cells were also examined. Results: B-RAF^V600E melanoma cells selected for resistant to PLX4720-induced apoptosis retained the V600E mutation in B-RAF, and proliferated steadily in the presence of the inhibitor, albeit with slow growth rate. These cells displayed high levels of ERK activation, that is, at least in part, independent of the conventional RAF/MEK/ERK pathway, as MEK activation was low and inhibition of MEK did not significantly block activation of ERK. In contrast, extracellular signals appeared involved. This was associated with elevated activation of the phosphoinositide 3-kinase (PI3k)/Akt pathway and could be inhibited by serum starvation and inhibition of PI3k/Akt. Inhibition of MEK did not impact on survival of these cells, whereas serum starvation, inhibition of PI3K/Akt, and inhibition of ERK1/2 reduced their viability. Conclusions: These results indicate that sensitivity to induction of apoptosis may be a major determinant of long-term responses of B-RAF^V600E melanomas to specific inhibitors and suggest that rebound melanoma growth after initial treatment with the inhibitors may not be responsive to MEK inhibitors, but may be susceptible to inhibition of the PI3k/Akt pathway.]]> Wed 11 Apr 2018 16:15:39 AEST ]]> Evidence for upregulation of Bim and the splicing factor SRp55 in melanoma cells from patients treated with selective BRAF inhibitors https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15167 Wed 11 Apr 2018 16:01:18 AEST ]]> MicroRNA-149*, a p53-responsive microRNA, functions as an oncogenic regulator in human melanoma https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:13811 Wed 11 Apr 2018 15:41:21 AEST ]]> 2-Deoxy-D-glucose enhances TRAIL-induced apoptosis in human melanoma cells through XBP-1-mediated up-regulation of TRAIL-R2 https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:6913 Wed 11 Apr 2018 15:38:21 AEST ]]> Effects of glucocorticoid exposure on growth and structural maturation of the heart of the preterm piglet https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:16610 Wed 11 Apr 2018 15:23:31 AEST ]]> Understanding the inflammatory mechanisms that predispose to emphysema in mouse models https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:10359 Wed 11 Apr 2018 15:22:31 AEST ]]> Apoptosis and DNA damage in human spermatozoa https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:23215 Wed 11 Apr 2018 15:05:01 AEST ]]> Apoptosis of human melanoma cells induced by inhibition of B-RAFᵛ⁶⁰⁰ᴱ involves preferential splicing of bimS https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:9452 Wed 11 Apr 2018 14:22:54 AEST ]]> The role of reactive oxygen species and oxidative stress in post-ovulatory ageing and apoptosis of the mammalian oocyte https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:22037 Wed 11 Apr 2018 14:21:26 AEST ]]> Differential roles of HOW in male and female Drosophila germline differentiation https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:15689 Wed 11 Apr 2018 13:43:52 AEST ]]> OBATOCLAX and ABT-737 induce ER stress responses in human melanoma cells that limit induction of apoptosis https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:14361 Wed 11 Apr 2018 13:27:51 AEST ]]> Mechanisms underlying 3-bromopyruvate-induced cell death in colon cancer https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:22877 Wed 11 Apr 2018 13:19:11 AEST ]]> Regulation of endoplasmic reticulum stress induced Apoptosis in human melanoma https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:2753 Wed 11 Apr 2018 13:06:05 AEST ]]> The role of altered nucleotide excision repair and UVB-induced DNA damage in melanomagenesis https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:12520 Wed 11 Apr 2018 12:41:53 AEST ]]> Inhibition of autophagy by 3-MA enhances endoplasmic reticulum stress-induced apoptosis in human nasopharyngeal carcinoma cells https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:14362 Wed 11 Apr 2018 12:28:38 AEST ]]> Targeting adaptative mechanisms to endoplasmic reticulum stress in melanoma https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:14172 Wed 11 Apr 2018 11:56:01 AEST ]]> P53 in human melanoma fails to regulate target genes associated with apoptosis and the cell cycle and may contribute to proliferation https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:13895 Wed 11 Apr 2018 11:47:28 AEST ]]> Expression of the uncharacterised isoform, BCL2β, in melanoma https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:27441 Wed 11 Apr 2018 11:10:42 AEST ]]> Inhibitory effects of 3-bromopyruvate in human nasopharyngeal carcinoma cells https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:26547 in vitro and in vivo. PI staining showed significant apoptosis in NPC cells accompanied by the overproduction of ROS and downregulation of mitochondrial membrane potential (MMP, ΔΨm) by 3-BrPA. However, the ROS scavenger N-acetyl-L-cysteine (NAC) significantly reduced 3-BrPA-induced apoptosis by decreasing ROS and facilitating the recovery of MMP. We elucidated the molecular mechanisms underlying 3-BrPA activity and found that it caused mitochondrial dysfunction and ROS production, leading to necroptosis of NPC cells. We investigated the effects of the caspase inhibitor z-VAD-fmk, which inhibits apoptosis but promotes death domain receptor (DR)-induced NPC cell necrosis. Necrostatin-1 (Nec-1) inhibits necroptosis, apparently via a DR signaling pathway and thus abrogates the effects of z-VAD‑fmk. In addition, we demonstrated the effective attenuation of 3-BrPA-induced necrotic cell death by Nec-1. Finally, animal studies proved that 3-BrPA exhibited significant antitumor activity in nude mice. The present study is the first demonstration of 3-BrPA-induced non-apoptotic necroptosis and ROS generation in NPC cells and provides potential strategies for developing agents against apoptosis‑resistant cancers.]]> Wed 11 Apr 2018 11:05:32 AEST ]]> Up-regulation of Mcl-1 is critical for survival of human melanoma cells upon endoplasmic reticulum stress https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:5046 Wed 11 Apr 2018 10:25:08 AEST ]]> Are sperm capacitation and apoptosis the opposite ends of a continuum driven by oxidative stress? https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:27621 Wed 11 Apr 2018 10:16:30 AEST ]]> Treatment combinations targeting apoptosis to improve immunotherapy of melanoma https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:6917 Wed 11 Apr 2018 09:56:34 AEST ]]> 5-Fluorouracil-induced apoptosis in colorectal cancer cells is caspase-9-dependent and mediated by activation of protein kinase C-d https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:18028 Wed 11 Apr 2018 09:44:48 AEST ]]> BCL-2 family isoforms in apoptosis and cancer https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:36748 Wed 10 Nov 2021 15:04:41 AEDT ]]> Non-benzoquinone geldanamycin analogs trigger various forms of death in human breast cancer cells https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:30132 Wed 09 Feb 2022 15:54:15 AEDT ]]> MAPK pathway: a potential target for the treatment of non-small-cell lung carcinoma https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:48577 Tue 21 Mar 2023 17:30:00 AEDT ]]> The protective mechanism underlying total flavones of Dracocephalum (TFD) effects on rat cerebral ischemia reperfusion injury https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:36238 Tue 17 Mar 2020 12:25:30 AEDT ]]> Cellular responses in titanium dioxide nanoparticle cytotoxicity studies: parts of the map waiting to be composed https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:30397 Tue 01 May 2018 09:18:50 AEST ]]> Acute cigarette smoke exposure activates apoptotic and inflammatory programs but a second stimulus is required to induce epithelial to mesenchymal transition in COPD epithelium https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:30666 Thu 28 Oct 2021 13:04:12 AEDT ]]> Knockdown of GRP78 enhances cell death by cisplatin and radiotherapy in nasopharyngeal cells https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:24957 in vivo and assessed for possible clinical applications.]]> Thu 27 Jan 2022 15:58:35 AEDT ]]> Flavonoid genistein protects bone marrow sinusoidal blood vessels from damage by methotrexate therapy in rats https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:38163 Thu 24 Aug 2023 15:10:03 AEST ]]> Leptospermum extract (QV0) suppresses pleural mesothelioma tumor growth in vitro and in vivo by mitochondrial dysfunction associated apoptosis https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:52625 Thu 19 Oct 2023 15:04:22 AEDT ]]> The bispidinone derivative 3,7-Bis-[2-(S)-amino-3-(1H-indol-3-yl)-propionyl]-1,5-diphenyl-3,7-diazabicyclo[3.3.1]nonan-9-one dihydrochloride induces an apoptosis-mediated cytotoxic effect on pancreatic cancer cells in vitro https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:34760 BisP1: 1,5-diphenyl-3,7-bis(2-hydroxyethyl)-3,7-diazabicyclo[3.3.1]nonan-9-one; BisP2: 3,7-bis-(2-(S)-amino-4-methylsulfanylbutyryl)-1,5-diphenyl-3,7-diazabicyclo[3.3.1]nonan-9-one dihydrochloride; BisP3: [2-{7-[2-(S)-tert-butoxycarbonylamino-3-(1H-indol-3-yl)-propionyl]-9-oxo-1,5-diphenyl-3,7-diazabicyclo[3.3.1]non-3-yl}-1-(S)-(1H-indol-3-ylmethyl)-2-oxoethyl]-carbamic acid tertbutyl ester; BisP4: 3,7-bis-[2-(S)-amino-3-(1H-indol-3-yl)-propionyl]-1,5-diphenyl-3,7-diazabicyclo[3.3.1]nonan-9-one dihydrochloride) was assessed against PC cell lines (MiaPaca-2, CFPAC-1 and BxPC-3). Cell viability was assessed using a Cell Counting Kit-8 (CCK-8) colorimetric assay, while apoptotic cell death was confirmed using fluorescence microscopy and flow cytometry. Initial viability screening revealed significant cytotoxic activity from BisP4 treatment (1 µM⁻100 µM) on all three cell lines, with IC50 values for MiaPaca-2, BxPC-3, and CFPAC-1 16.9 µM, 23.7 µM, and 36.3 µM, respectively. Cytotoxic treatment time-response (4 h, 24 h, and 48 h) revealed a 24 h treatment time was sufficient to produce a cytotoxic effect on all cell lines. Light microscopy evaluation (DAPI staining) of BisP4 treated MiaPaca-2 PC cells revealed dose-dependent characteristic apoptotic morphological changes. In addition, flow cytometry confirmed BisP4 induced apoptotic cell death induction of activated caspase-3/-7. The bispidinone derivative BisP4 induced an apoptosis-mediated cytotoxic effect on MiaPaca-2 cell lines and significant cytotoxicity on CFPAC-1 and BxPC-3 cell lines. Further investigations into the precise cellular mechanisms of action of this class of compounds are necessary for potential development into pre-clinical trials.]]> Thu 17 Mar 2022 14:34:42 AEDT ]]> Identification of miR-145-5p-centered competing endogenous RNA network in laryngeal squamous cell carcinoma https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:36692 Thu 13 Jan 2022 10:31:57 AEDT ]]> Melatonin alleviates heat stress-induced oxidative stress and apoptosis in human spermatozoa https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:49319 Thu 11 May 2023 14:53:05 AEST ]]> Eucalyptus microcorys leaf extract derived HPLC-fraction reduces the viability of MIA PaCa-2 cells by inducing apoptosis and arresting cell cycle https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:33409 Eucalyptus microcorys extract against pancreatic cancer cell lines. In this study, bioassay-guided fractionation of the aqueous crude E. microcorys extract using RP-HPLC and subsequent assessment of the resultant fractions (F1-F5) for their antioxidant activity and cytotoxicity against pancreatic cancer cell lines were performed. The molecular mechanisms associated with the cytotoxicity was characterised by studying the effects of the most potent fraction-1 (F1) on apoptosis and cell cycle profiles as well as its phytochemical constituents by LC-ESI/MS/MS. F1 displayed significantly greater antioxidant activity in three different assays (p < 0.05). Moreover, F1 exhibited significantly greater antiproliferative activity (IC₅₀ = 93.11 ± 3.43 µg/mL) against MIA PaCa-2 cells compared to the other four fractions (p < 0.05). F1 induced apoptosis by regulating key apoptotic proteins- Bcl-2, Bak, Bax, cleaved PARP, procaspase-3 and cleaved caspase-3 in MIA PaCa-2 cells, suggesting the involvement of intrinsic mitochondrial apoptotic pathway and arrested cells at G2/M phase. A combination of gemcitabine and F1 exerted a greater effect on apoptosis and cell cycle arrest than F1 or gemcitabine alone (p < 0.05). LC-ESI/MS/MS revealed the tentative identities of phytochemicals present in F1 and their similarities with the phenolic compounds previously reported in Eucalyptus with antipancreatic cancer activity. Our study shows that the polyphenol and antioxidant-rich fraction of E. microcorys extract is a promising candidate for developing mono or combination therapies against pancreatic cancer.]]> Thu 09 Dec 2021 11:03:58 AEDT ]]> Targeting apoptotic pathways in acute myeloid leukaemia https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:44081 Thu 06 Oct 2022 15:06:26 AEDT ]]> An array of bioactive compounds from Australian eucalypts and their relevance in pancreatic cancer therapeutics https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:33408 Thu 04 Nov 2021 10:39:25 AEDT ]]> Methylmercury neurotoxicity is associated with inhibition of the antioxidant enzyme glutathione peroxidase https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:7232 Sat 24 Mar 2018 08:40:26 AEDT ]]> Inhibition of MEK blocks GRP78 up-regulation and enhances apoptosis induced by ER stress in gastric cancer cells https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:7349 Sat 24 Mar 2018 08:40:16 AEDT ]]> Emerging role of tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) as a key regulator of inflammatory responses https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:7083 Sat 24 Mar 2018 08:37:59 AEDT ]]> Human melanoma cells under endoplasmic reticulum stress acquire resistance to microtubule-targeting drugs through XBP-1-mediated activation of Akt https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:7006 Sat 24 Mar 2018 08:37:51 AEDT ]]> Human melanoma cells under endoplasmic reticulum stress are more susceptible to apoptosis induced by the BH3 mimetic obatoclax https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:7007 Sat 24 Mar 2018 08:37:51 AEDT ]]> Inhibition of MEK sensitizes paclitaxel-induced apoptosis of human colorectal cancer cells by downregulation of GRP78 https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:8335 Sat 24 Mar 2018 08:37:01 AEDT ]]> Exercise and supraphysiological dose of nandrolone deconoate increase apoptosis in spermatogenic cells https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:9623 Sat 24 Mar 2018 08:35:27 AEDT ]]> TRAIL-induced apoptosis of human melanoma cells involves activation of caspase-4 https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:9621 Sat 24 Mar 2018 08:35:27 AEDT ]]> On the possible origins of DNA damage in human spermatozoa https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:9578 Sat 24 Mar 2018 08:34:48 AEDT ]]> Granzyme B deficiency exacerbates lung inflammation in mice after acute lung injury https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:14651 Sat 24 Mar 2018 08:20:46 AEDT ]]> Targeting anti-apoptotic mechanisms for reversal of resistance to BRAF inhibitors in melanoma https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:13255 Sat 24 Mar 2018 08:16:00 AEDT ]]> Factors affecting cytotrophoblast cell viability and differentiation: evidence of a link between syncytialisation and apoptosis https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:12438 Sat 24 Mar 2018 08:15:27 AEDT ]]> Human neuroblastoma cells transfected with tyrosine hydroxylase gain increased resistance to methylmercury-induced cell death https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:10760 Sat 24 Mar 2018 08:13:54 AEDT ]]> Hybrid promoters directed tBid gene expression to breast cancer cells by transcriptional targeting https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:10698 Sat 24 Mar 2018 08:09:55 AEDT ]]> Novel non-cyclooxygenase inhibitory derivatives of naproxen for colorectal cancer chemoprevention https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:19006 50 of 11.4 μM (two orders of magnitude more potent than naproxen). Selectivity of 3a was investigated against a panel of three tumor and one normal colon cell lines and showed up to six times less toxicity against normal colonocytes. Compound 3a was shown to induce dose-dependent apoptosis of HT116 colon tumor cells as evidenced by measuring the activity of caspases-3 and 7. None of the synthesized compounds showed activity against COX-1 or COX-2 isozymes, confirming a COX-independent mechanism of action. Compound 3k was found to have no ulcerogenic effect in rats as indicated by electron microscope scanning of the stomach after oral administration. A pharmacophore model was developed for elucidating structure–activity relationships and subsequent chemical optimization for this series of compounds as colorectal cancer chemopreventive drugs.]]> Sat 24 Mar 2018 08:05:35 AEDT ]]> The John Hughes Memorial Lecture: aspects of sperm physiology-oxidative stress and the functionality of stallion spermatozoa https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:20539 Sat 24 Mar 2018 08:02:43 AEDT ]]> Inhibition of MEK sensitizes gastric cancer cells to TRAIL-induced apoptosis https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:20568 Sat 24 Mar 2018 08:02:38 AEDT ]]> Methoxyphenylcipro induces antitumor activity in human cancer cells https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:17312 Sat 24 Mar 2018 08:01:52 AEDT ]]> Down-regulation of RIP1 by 2-deoxy-D-glucose sensitizes breast cancer cells to TRAIL-induced apoptosis https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:17365 Sat 24 Mar 2018 08:01:31 AEDT ]]> Melatonin prevents postovulatory oocyte aging in the mouse and extends the window for optimal fertilization in vitro https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:20252 Sat 24 Mar 2018 07:59:55 AEDT ]]> Analysis of the global methylation status of human spermatozoa and its association with the tendency of these cells to enter apoptosis https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:19943 Sat 24 Mar 2018 07:58:35 AEDT ]]> Causes and consequences of apoptosis in spermatozoa; contributions to infertility and impacts on development https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:19949 Sat 24 Mar 2018 07:58:32 AEDT ]]> 3-Bromopyruvate induces apoptosis in breast cancer cells by downregulating Mcl-1 through the PI3K/Akt signaling pathway https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:18033 Sat 24 Mar 2018 07:56:32 AEDT ]]> Phosphoinositide 3-kinase signalling pathway involvement in a truncated apoptotic cascade associated with motility loss and oxidative DNA damage in human spermatozoa https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:17890 Sat 24 Mar 2018 07:56:25 AEDT ]]> Apoptosis in the germ line https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:17960 Sat 24 Mar 2018 07:56:21 AEDT ]]> Equilibria and stability of a class of positive feedback loops: mathematical analysis and its application to caspase-dependent apoptosis https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:19688 Sat 24 Mar 2018 07:53:50 AEDT ]]> Inhibition of apoptosis facilitates necrosis induced by cisplatin in gastric cancer cells https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:5563 Sat 24 Mar 2018 07:49:11 AEDT ]]> Activation of Jun N-terminal kinase is a mediator of vincristine-induced apoptosis of melanoma cells https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:5683 Sat 24 Mar 2018 07:47:32 AEDT ]]> Overcoming resistance to apoptosis in cancer therapy https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:6686 Sat 24 Mar 2018 07:46:12 AEDT ]]> Inhibition of endoplasmic reticulum stress-induced apoptosis of melanoma cells by the ARC protein https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:5319 Sat 24 Mar 2018 07:45:57 AEDT ]]> Involvement of endoplasmic reticulum stress in docetaxel-induced JNK-dependent apoptosis of human melanoma https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:5394 Sat 24 Mar 2018 07:43:57 AEDT ]]> Melanoma cell sensitivity to docetaxal-induced apoptosis is determined by class III β-tubulin levels https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:5398 Sat 24 Mar 2018 07:43:56 AEDT ]]> miR-323a-3p regulates lung fibrosis by targeting multiple profibrotic pathways https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:29820 Sat 24 Mar 2018 07:40:51 AEDT ]]> The effects of aging on apoptosis following myocardial infarction https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:28830 Sat 24 Mar 2018 07:38:24 AEDT ]]> The TrK receptor family https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:26338 NTR, sortilin) that can interact with the Trks and affect their activity. The Trks are predominantly expressed in nervous tissues, where they are essential for the growth, development, and maintenance of select types of both peripheral and central neurons and are involved in neurodegenerative diseases. The Trks are also found in some nonneuronal tissues and in a wide variety of tumors, where they can play an active role in tumor progression and metastasis. The activated Trk receptors primarily bind and signal through Shc, FRS2, and PLCᵧ, which in turn activate PI3K, the Erks, and the hydrolysis of inositol phospholipids, among other events, leading to the modulation of gene transcription.]]> Sat 24 Mar 2018 07:35:55 AEDT ]]> Targeting apoptotic pathways in melanoma https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:25800 Sat 24 Mar 2018 07:34:44 AEDT ]]> Damage to sperm DNA mediated by reactive oxygen species: its impact on human reproduction and the health trajectory of offspring https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:26609 Sat 24 Mar 2018 07:33:58 AEDT ]]> Altholactone induces apoptotic cell death in human colorectal cancer cells https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:28516 Sat 24 Mar 2018 07:29:20 AEDT ]]> Intervention of pentoxifylline on silica-induced inflammatory reaction and apoptosis https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:26721 Sat 24 Mar 2018 07:26:21 AEDT ]]> A mouse retinal explant model for use in studying neuroprotection in glaucoma https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:26685 ex-vivo. Explants were treated either with Z-VAD-FMK (a pan-caspase inhibitor; 100µM) or vehicle. Retinal Ganglion cell (RGC) density was analysed by βIII tubulin and RNA-binding protein with multiple splicing (RBPMS) immunohistochemistry. Caspase activity was measured using Caspase 3/7 glo assay and western blot. Caspase-3 expression was quantified using RT-PCR and western blotting. Retinal explants treated with Z-VAD-FMK demonstrated a 1.5-fold (p = 0.027) increase in number of surviving RGCs on day 4 compared to the control treatment using ßIII tubulin staining. RGC viability was 2-fold (p = 0.002) higher in RGC stained with RBPMS on day 1 compared to control. There was no RBPMS staining of RGCs beyond day 1 in either treatment. The caspase activity was 4.75 and 5.5-fold (p = 0.002 and 0.004 respectively) higher in control as compared to treatment with Z-VAD-FMK on day 1, 2 respectively. Increase in caspase activity in control group was also confirmed by western blot for day 1 protein lysates. Caspase-3 mRNA expression was 4.75-fold higher in Z-VAD-FMK treated explants compared to control on day 1 (p < 0.001). Culture conditions appropriate to retinal explant culture for investigation of RGC apoptosis was identified. Retinal cultures at day 4 were ideal for detecting neuroprotection using ßIII tubulin staining. RBPMS acts as a viability marker as well as to define best time point for investigation of apoptosis related signalling pathways which is day 1. These findings suggest that mouse retinal explants are good model for studying ganglion cell specific apoptosis and are applicable to diseases such as glaucoma.]]> Sat 24 Mar 2018 07:26:17 AEDT ]]> Phagoptosis: cell death by phagocytosis: plays central roles in physiology, host defense and pathology https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:26189 Sat 24 Mar 2018 07:24:10 AEDT ]]> Reactive oxygen species and sperm function-in sickness and in health https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:22254 Sat 24 Mar 2018 07:17:34 AEDT ]]> Autophagy-mediated HMGB1 release antagonizes apoptosis of gastric cancer cells induced by vincristine via transcriptional regulation of Mcl-1 https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:23419 Sat 24 Mar 2018 07:13:54 AEDT ]]> The melanoma-associated antigen MAGE-D2 suppresses TRAIL receptor 2 and protects against TRAIL-induced apoptosis in human melanoma cells https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:23422 Sat 24 Mar 2018 07:13:54 AEDT ]]> Involvement of homocysteine, homocysteine thiolactone, and paraoxonase type 1 (PON-1) in the etiology of defective human sperm function https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:24493 Sat 24 Mar 2018 07:13:10 AEDT ]]> Electrophilic aldehydes generated by sperm metabolism activate mitochondrial reactive oxygen species generation and apoptosis by targeting succinate dehydrogenase https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:22207 R²= 0.89) in untreated populations of human spermatozoa emphasized the pathophysiological significance of these findings. The latter also provide a biochemical explanation for the self-perpetuating nature of oxidative stress in the male germ line, with the products of lipid peroxidation stimulating free radical generation by the sperm mitochondria in a positive feedback loop.]]> Sat 24 Mar 2018 07:11:16 AEDT ]]> 17-demethoxy-reblastatin, an Hsp90 inhibitor, induces mitochondria-mediated apoptosis through downregulation of Mcl-1 in human hepatocellular carcinoma cells https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:22689 Streptomyces hygroscopicus. In the present study, we examined the anticancer effects of 17-DR on human hepatocellular carcinoma (HCC) cell lines HepG2 and SMMC7721, and its underlying mechanisms. The results indicated that 17-DR could concentration-dependently inhibit the proliferation, and decrease the colony formation in HCC cells. It also induced significant apoptosis in HCC cells, which was mediated by mitochondria via a caspase-dependent pathway. The mechanisms involved in 17-DR-induced apoptosis included the downregulation of myeloid cell leukemia-1 (Mcl-1), and upregulation of Bcl-2 antagonist killer 1 (Bak). And the upregulated Bak expression resulted from downregulation of Mcl-1 played an essential role in this process. Taken together, these results indicated that 17-DR possessed potent anticancer effects on HCC cells by inhibiting cell proliferation and inducing apoptosis.]]> Sat 24 Mar 2018 07:11:11 AEDT ]]> Can hemp help? Low-THC cannabis and non-THC cannabinoids for the treatment of cancer https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:38260 9-tetrahydrocannabinol (∆⁹-THC). Evidence is emerging for the therapeutic benefits of cannabis in the treatment of neurological and neurodegenerative diseases, with potential efficacy as an analgesic and antiemetic for the management of cancer-related pain and treatment-related nausea and vomiting, respectively. An increasing number of preclinical studies have established that ∆⁹-THC can inhibit the growth and proliferation of cancerous cells through the modulation of cannabinoid receptors (CB1R and CB2R), but clinical confirmation remains lacking. In parallel, the anti-cancer properties of non-THC cannabinoids, such as cannabidiol (CBD), are linked to the modulation of non-CB1R/CB2R G-protein-coupled receptors, neurotransmitter receptors, and ligand-regulated transcription factors, which together modulate oncogenic signalling and redox homeostasis. Additional evidence has also demonstrated the anti-inflammatory properties of cannabinoids, and this may prove relevant in the context of peritumoural oedema and the tumour immune microenvironment. This review aims to document the emerging mechanisms of anti-cancer actions of non-THC cannabinoids.]]> Mon 29 Jan 2024 17:45:13 AEDT ]]> Involvement of apoptosis in the protective effects of Dracocephalum moldavaica in cerebral ischemia reperfusion rat model https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:46650 Dracocephalum moldevica (DML) was found to exert protective effects on cerebral ischemia-reperfusion injury (CIRI); however, the mechanisms underlying the observed actions of this plant-derived mixture remain to be determined. Thus, the aim of this study was to examine the influence of DML on CIRI rat model induced by middle cerebral artery occlusion (MCAO). The following parameters were measured: (1) viable neurons in the infarcted area using Nissl staining; and (2) immunohistochemistry and Western blot were employed to determine protein expression levels of p53, bcl-2 associated X protein (bax) and B-cell lymphoma-2 (bcl-2), three biomarkers of apoptosis. MCAO significantly decreased the number of viable cortical pyramidal neurons in the infarcted area, while treatment with DML extract significantly elevated the number of viable neurons. MCAO was found to significantly elevate in gene expression levels of p53 and protein expression levels bax accompanied by diminished protein expression levels of bcl-2. Prior administration of DML extract produced marked reduction in gene expression levels of p53 and protein expression levels bax but increased in protein expression levels of bcl-2. Data suggested apoptosis was initiated in MCAO and that DML was effective in treating CIRI via an anti-apoptotic action as evidenced by inhibition of gene expression levels of p53 and protein expression levels of bax with concomitant elevation in protein expression levels of bcl-2. Our findings suggest that extract of DML may prove beneficial in treatment of cerebrovascular disorders.]]> Mon 28 Nov 2022 17:22:23 AEDT ]]> Contribution of cell death to the pathogenesis of chronic obstructive pulmonary disease (COPD) https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:36167 Mon 26 Oct 2020 11:55:19 AEDT ]]> Peptide-Conjugated Micelles Make Effective Mimics of the TRAIL Protein for Driving Apoptosis in Colon Cancer https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:54743 Mon 11 Mar 2024 14:25:43 AEDT ]]> Evidence that extrapancreatic insulin production is involved in the mediation of sperm survival https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:40400 Mon 11 Jul 2022 13:55:46 AEST ]]> Phytochemicals derived from Australian eucalypts as anticancer agents for pancreatic malignancies https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:32711 Fri 20 Sep 2019 02:27:30 AEST ]]> The upregulation of stromal antigen 3 expression suppresses the phenotypic hallmarks of hepatocellular carcinoma through the Smad3-CDK4/CDK6-cyclin D1 and CXCR4/RhoA pathways https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:52684 Fri 20 Oct 2023 09:58:07 AEDT ]]> The protective mechanisms underlying ginsenoside Rg1 effects on rat sciatic nerve injury https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:42217 Fri 19 Aug 2022 11:21:32 AEST ]]> Phyllanthus emblica fruit extract attenuates lipid metabolism in 3T3-L1 adipocytes via activating apoptosis mediated cell death https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:46116 Phyllanthus emblica L. (Indian gooseberry) is widely used in the Ayurveda for thousands of years to treat health complications including disorders of the immune system, diabetes, and obesity. Purpose: For the first time, our study aims to demonstrate the molecular mechanisms of the fruit extract of Phyllanthus emblica (PEFE) involved in the promotion of fat cell apoptosis and alleviation of adipogenesis. Methods: The active constituents from PEFE were identified using high performance liquid chromatography-mass spectrometry (HPLC-MS). We carried out the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay to evaluate the cytotoxic effects of PEFE using 3T3-L1 pre-adipocytes. The colonogenic assay was carried out to determine the inhibitory effect of 3T3-L1 adipocytes after PEFE treatment. In addition, inhibition of pancreatic lipase activity was performed and the lipolytic activity of PEFE and digallic acid was compared with the well-known standard drug orlistat. Besides, the molecular interaction and ligand optimization between digallic and adipogenesis/apoptosis markers were also carried out. Furthermore, to confirm fat cell apoptosis we have used several detection methods that includes Hoechst staining, PI staining, Oil staining and qPCR respectively. Results: Digallic acid was identified as a major component in the PEFE. The IC₅₀ values of digallic acid and PEFE were found to be 3.82 µg/ml and 21.85 µg/ml respectively. PEFE and digallic acid showed significant anti-lipolytic activity compared to the standard drug orlistat. In the mature adipocytes, PEFE significantly decreased triglyceride accumulation by downregulating adiponectin, PPARγ, cEBPα, and FABP4 respectively. We further analyzed the expression of apoptosis related genes upon PEFE treatment. Apoptotic process initiated through upregulation of BAX and downregulation of BCL2 resulting in an increased caspase-3 activity. In addition, we have also confirmed the apoptosis and DNA fragmentation in 3T3-L1 cells using Hoechst, PI and TUNEL assays. Conclusion: PEFE negatively regulates adipogenesis by initiating fat cell apoptosis and therefore it can be considered as a potential herbal medicinal product for treating obesity.]]> Fri 11 Nov 2022 14:46:51 AEDT ]]> Elaeocarpus reticulatus fruit extracts reduce viability and induce apoptosis in pancreatic cancer cells in vitro https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:37726 Fri 03 Dec 2021 10:33:48 AEDT ]]>