https://novaprd-lb.newcastle.edu.au/vital/access/manager/Index ${session.getAttribute("locale")} 5 Degradation of APCcdc²⁰ and APCcdh¹ substrates during the second meiotic division in mouse eggs https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:6544 Wed 11 Apr 2018 16:31:09 AEST ]]> Identification of sites of STAT3 action in the female reproductive tract through conditional gene deletion https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:16815 fl/fl with Zp3-cre+ mice, or in ovarian granulosa cells and uterine stroma by crossing with Amhr2-Cre+ mice. Surprisingly, deletion of STAT3 in oocytes had no effect on fertility indicating that the abundance of STAT3 protein in maturing oocytes and fertilized zygotes is not essential to these developmental stages. In Stat3fl/fl;Amhr2-cre+ females impaired fertility was observed through significantly fewer litters and smaller litter size. Ovulation rate, oocyte fertilization and development to blastocyst were unaffected in this line; however, poor recombination efficiency in granulosa cells had yielded no net change in STAT3 protein abundance. In contrast, uteri from these mice showed STAT3 protein depletion selectively from the stomal compartment. A significant reduction in number of viable fetuses on gestational day 18, increased fetal resorptions and disrupted placental morphology were evident causes of the reduced fertility. In conclusion, this study defines an important role for STAT3 in uterine stromal cells during embryo implantation and the development of a functional placenta.]]> Wed 11 Apr 2018 15:06:51 AEST ]]> Kif4 is essential for mouse oocyte meiosis https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:30985 Wed 11 Apr 2018 14:34:15 AEST ]]> APC FZR1 prevents nondisjunction in mouse oocytes by controlling meiotic spindle assembly timing https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:18262 Wed 11 Apr 2018 11:37:00 AEST ]]> The APC activator fizzy-related-1 (FZR1) is needed for preimplantation mouse embryo development https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:21729 Fzr1 knockout mouse embryos show normal preimplantation development but die due to a lack of endoreduplication needed for placentation. However, interpretation of the role of FZR1 during this period is hindered by the presence of maternal stores. In this study, therefore, we used an oocyte-specific knockout to examine FZR1 function in early mouse embryo development. Maternal FZR1 was not crucial for completion of meiosis, and furthermore viable pups were born to Fzr1 knockout females mated with normal males. However, in early embryos the absence of both maternal and paternal FZR1 led to a dramatic loss in genome integrity, such that the majority of embryos arrested having undergone only a single mitotic division and contained many γ-H2AX foci, consistent with fragmented DNA. A prominent feature of such embryos was the establishment of two independent spindles following pronuclear fusion and thus a failure of the chromosomes to mix (syngamy). These generated binucleate 2-cell embryos. In the 10% of embryos that progressed to the 4-cell stage, division was so slow that compaction occurred prematurely. No embryo development to the blastocyst stage was ever observed. We conclude that Fzr1 is a surprisingly essential gene involved in the establishment of a single spindle from the two pronuclei in 1-cell embryos as well as being involved in the maintenance of genomic integrity during the mitotic divisions of early mammalian embryos.]]> Wed 11 Apr 2018 11:35:19 AEST ]]> Control of chromosome segregation in mouse oocytes https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:11275 Wed 11 Apr 2018 09:23:59 AEST ]]> Start and stop signals of oocyte meiotic maturation https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:18182 Tue 23 Jun 2015 18:36:00 AEST ]]> Sucrose transporter localization and function in phloem unloading in developing stems https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:33602 Sorghum bicolor SUTs SbSUT1 and SbSUT5 were characterized by determining their transport properties heterologously expressed in yeast or Xenopus laevis oocytes, and their in planta cellular and subcellular localization. The plasma membrane-localized SbSUT1 and SbSUT5 exhibited a strong selectivity for Suc and high Suc affinities in X. laevis oocytes at pH 5—SbSUT1, 6.3 ± 0.7 mm, and SbSUT5, 2.4 ± 0.5 mm Suc. The Suc affinity of SbSUT1 was dependent on membrane potential and pH. In contrast, SbSUT5 Suc affinity was independent of membrane potential and pH but supported high transport rates at neutral pH. Suc transport by the tonoplast localized SbSUT4 could not be detected using yeast or X. laevis oocytes. Across internode development, SUTs, other than SbSUT4, were immunolocalized to sieve elements, while for elongating and recently elongated internodes, SUTs also were detected in storage parenchyma cells. We conclude that apoplasmic Suc unloading from de-energized protophloem sieve elements in meristematic zones may be mediated by reversal of SbSUT1 and/or by uniporting SWEETs. Storage parenchyma localized SbSUT1 and SbSUT5 may accumulate Suc from the stem apoplasms of elongating and recently elongated internodes, whereas SbSUT4 may function to release Suc from vacuoles. Transiting from an apoplasmic to symplasmic unloading pathway as the stem matures, SbSUT1 and SbSUT5 increasingly function in Suc retrieval into metaphloem sieve elements to maintain a high turgor to drive symplasmic unloading by bulk flow.]]> Thu 22 Nov 2018 16:43:24 AEDT ]]> Oxidative damage in naturally aged mouse oocytes is exacerbated by dysregulation of proteasomal activity https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:43344 Thu 15 Sep 2022 15:19:01 AEST ]]> Dissociation and preservation of preantral follicles and immature oocytes from female dasyurid marsupials https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:7747 Sat 24 Mar 2018 08:41:50 AEDT ]]> Comparison of the production, quality, and in vitro maturation capacity of oocytes from untreated cycling and intermediate phase equine serum gonadotropin-treated fat-tailed dunnarts (Sminthopsis crassicaudata) https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:7794 12 month) fat-tailed dunnarts, Sminthopsis crassicaudata. Increased urinary cornified epithelial cells and the influx of leukocytes defined day 0, at which time the naturally cycling females had already ovulated; at day 16 females had no antral follicles, but by day 20 antral follicles had begun to develop. There was no difference between naturally cycling breeding and retired females. Females were stimulated with 1 IU equine serum gonadotropin (eSG) during the intermediate phase on day 16 and killed 3, 4, or 5 days later. Stimulation resulted in a significant increase in the number of growing antral follicles but retired females demonstrated a reduced response. Upon collection from breeding females 4 days following eSG stimulation, 100% of oocytes were at the first polar body (PB1) stage, those collected from retired females were immature upon collection but within 48 h 98.2±1.9% were cultured to the PB1 stage. The rate of ovulation was high in breeding females 5 days following stimulation but retired females were less reliable, and in both groups all oocytes were degraded. This is the first study to describe a reliable technique, involving ovarian stimulation during the intermediate phase and segregation of age groups, allowing the collection of a large number of healthy PB1 stage oocytes from S. crassicaudata. This is important for the development of further assisted reproductive techniques for this species and threatened dasyurids.]]> Sat 24 Mar 2018 08:39:18 AEDT ]]> Awakening the oocyte: controlling primordial follicle development https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:7505 Sat 24 Mar 2018 08:38:29 AEDT ]]> Contraceptive vaccines https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:1851 Sat 24 Mar 2018 08:31:16 AEDT ]]> On regenerating the ovary and generating controversy https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:1735 Sat 24 Mar 2018 08:27:24 AEDT ]]> Successful recovery of motility and fertility of cryopreserved cane toad (Bufo marinus) sperm https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:2805 Sat 24 Mar 2018 08:27:19 AEDT ]]> The Aurora kinase inhibitor ZM447439 accelerates first meiosis in mouse oocytes by overriding the spindle assembly checkpoint https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:11469 Sat 24 Mar 2018 08:11:09 AEDT ]]> Non-canonical function of spindle assembly checkpoint proteins after APC activation reduces aneuploidy in mouse oocytes https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:21277 Sat 24 Mar 2018 07:54:41 AEDT ]]> Injections of porcine sperm extracts trigger fertilization-like calcium oscillations in oocytes of a marine worm https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:6580 Sat 24 Mar 2018 07:49:18 AEDT ]]> Maintenance of sister chromatid attachment in mouse eggs through maturation-promoting factor activity https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:6574 Sat 24 Mar 2018 07:49:16 AEDT ]]> Sperm-induced Ca²⁺ oscillations in mouse oocytes and eggs can be mimicked by photolysis of caged inositol 1,4,5-trisphosphate: evidence to support a continuous low level production of inositol 1,4,5-trisphosphate during mammalian fertilization https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:6576 Sat 24 Mar 2018 07:49:16 AEDT ]]> Meiotic and mitotic Ca²⁺ oscillations affect cell composition in resulting blastocysts https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:6585 Sat 24 Mar 2018 07:49:10 AEDT ]]> A comparison of sperm- and IP₃-induced Ca²⁺ release in activated and aging mouse oocytes https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:6584 Sat 24 Mar 2018 07:49:10 AEDT ]]> Unique protein kinase C profile in mouse oocytes: lack of calcium-dependent conventional isoforms suggested by rtPCR and Western blotting https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:6586 Sat 24 Mar 2018 07:49:07 AEDT ]]> The CRY box: a second APCcdh¹-dependent degron in mammalian cdc20 https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:6556 Sat 24 Mar 2018 07:48:22 AEDT ]]> Prometaphase APCcdh¹ activity prevents non-disjunction in mammalian oocytes https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:6537 Sat 24 Mar 2018 07:48:19 AEDT ]]> Ionomycin, thapsigargin, ryanodine, and sperm induced Ca²⁺ release increase during meiotic maturation of mouse oocytes https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:6541 Sat 24 Mar 2018 07:48:19 AEDT ]]> APCcdh¹ activity in mouse oocytes prevents entry into the first meiotic division https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:6563 Sat 24 Mar 2018 07:47:07 AEDT ]]> Essential CDK1-inhibitory role for separase during meiosis I in vertebrate oocytes https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:6562 Sat 24 Mar 2018 07:47:07 AEDT ]]> Differential regulation of cyclin B1 degradation between the first and second meiotic divisions of bovine oocytes https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:25109 Sat 24 Mar 2018 07:17:14 AEDT ]]> Anaphase-promoting complex control in female mouse meiosis https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:23214 Sat 24 Mar 2018 07:10:28 AEDT ]]> OXIDATIVE STRESS AND REPRODUCTIVE FUNCTION: Oxidative stress and in vitro ageing of the post-ovulatory oocyte: an update on recent advances in the field https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:51259 Mon 28 Aug 2023 13:50:03 AEST ]]> Quality control mechanisms responsible for the maintenance of genomic integrity in the female germline https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:33931 Mon 23 Sep 2019 13:28:19 AEST ]]>