https://novaprd-lb.newcastle.edu.au/vital/access/manager/Index ${session.getAttribute("locale")} 5 Mechanisms of tethering and cargo transfer during epididymosome-sperm interactions https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:45249 Wed 26 Oct 2022 19:43:42 AEDT ]]> Marsupial sperm antigens https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:37295 Wed 18 Nov 2020 16:01:36 AEDT ]]> Molecular and functional characterization of the rabbit epididymal secretory protein 52, REP52 https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:4505 Wed 11 Apr 2018 16:03:28 AEST ]]> The role of molecular chaperones in spermatogenesis and the post-testicular maturation of mammalian spermatozoa https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:11890 Wed 11 Apr 2018 15:29:17 AEST ]]> Actin polymerisation during morphogenesis of the acrosome as spermatozoa undergo epididymal maturation in the tammar wallaby (Macropus eugenii) https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:1273 Wed 11 Apr 2018 14:07:43 AEST ]]> Identification of SRC as a key PKA-stimulated tyrosine kinase involved in the capacitation-associated hyperactivation of murine spermatozoa https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:1286 Wed 11 Apr 2018 11:40:47 AEST ]]> The 'omics revolution and our understanding of sperm cell biology https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:25253 Wed 11 Apr 2018 11:30:48 AEST ]]> Functional maturation of mouse epididymal spermatozoa https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:3122 Wed 11 Apr 2018 10:52:30 AEST ]]> Identification of the molecular chaperone, heat shock protein 1 (chaperonin 10), in the reproductive tract and in capacitating spermatozoa in the male mouse https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:4506 Wed 11 Apr 2018 10:11:11 AEST ]]> Post-testicular sperm maturation in the saltwater crocodile Crocodylus porosus: assessing the temporal acquisition of sperm motility https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:39495 Crocodylus porosus as a tractable model for understanding crocodilian sperm physiology. In extending our systematic characterisation of saltwater crocodile spermatozoa, in this study we examined the development of motility during sperm transport through the excurrent duct system of the male crocodile. The results show that approximately 20% of crocodile testicular spermatozoa are immediately motile but experience a gradient of increasing motility (percentage motile and rate of movement) as they transit the male reproductive tract (epididymis). Moreover, we confirmed that, as in ejaculated crocodile spermatozoa, increased intracellular cAMP levels promoted a significant and sustained enhancement of sperm motility regardless of whether the cells were isolated from the testis or epididymis. Along with the development of artificial reproductive technologies, this research paves the way for the opportunistic recovery, storage and potential utilisation of post-mortem spermatozoa from genetically valuable animals.]]> Tue 09 Aug 2022 14:34:34 AEST ]]> Molecular insights into the divergence and diversity of post-testicular maturation strategies https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:40953 Tue 07 Nov 2023 09:51:49 AEDT ]]> Developmental expression of the dynamin family of mechanoenzymes in the mouse epididymis https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:34016 Thu 30 May 2019 15:47:52 AEST ]]> Characterization of a novel role for the dynamin mechanoenzymes in the regulation of human sperm acrosomal exocytosis https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:34014 Thu 30 May 2019 15:41:20 AEST ]]> Identification of post-translational modifications that occur during sperm maturation using difference in two-dimensional gel electrophoresis https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:7 Thu 25 Jul 2013 09:10:12 AEST ]]> Mouse quiescin sulfhydryl oxidases exhibit distinct epididymal luminal distribution with segment-specific sperm surface associations https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:43294 Thu 15 Sep 2022 12:51:41 AEST ]]> New proteins identified in epididymal fluid from the platypus (Ornithorhynchus anatinus) https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:7848 Sat 24 Mar 2018 08:42:26 AEDT ]]> Proteomics of human spermatozoa https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:8464 Sat 24 Mar 2018 08:42:09 AEDT ]]> Mammalian epididymal proteome https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:7495 Sat 24 Mar 2018 08:37:44 AEDT ]]> Rabbit epididymal secretory proteins. I. Characterization and hormonal regulation https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:1978 region 7 > region 6 > region 1 > region 8 > ductuli efferentes), and orchidectomy had little effect on the rates. Incorporation into four proteins that were secreted in vitro (Mr 38 000, 20 000, 15 000, and 13 000) was reduced or abolished by orchidectomy and restored by testosterone therapy. The secretion of three proteins (Mr 52 000, 23 000, and 22 000) was reduced or abolished by orchidectomy and not restored by testosterone therapy. SDS-PAGE of detergent extracts of sperm indicated that five proteins were lost and nine were gained during epididymal transit. Seven of the proteins gained were about the same molecular weight as proteins secreted by the epididymis (Mr 94 000, 52 000, 38 000, 36 000, 22 000, 20 000, and 13 000) and were analyzed using N-terminal amino acid microsequencing.]]> Sat 24 Mar 2018 08:33:15 AEDT ]]> Acrosome formation during sperm transit through the epididymis in two marsupials, the tammar wallaby (Macropus eugenii) and the brushtail possum (Trichosurus vulpecula) https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:1076 Sat 24 Mar 2018 08:32:09 AEDT ]]> The development of signal transduction pathways during epididymal maturation is calcium dependent https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:2526 Sat 24 Mar 2018 08:31:07 AEDT ]]> Induction of sperm maturation in vitro in epididymal cell cultures of the tammar wallaby (Macropus eugenii): disruption of motility initiation and sperm morphogenesis by inhibition of actin polymerization https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:1517 Sat 24 Mar 2018 08:30:49 AEDT ]]> Tracing sperm acrosome differentiation in the testis and maturation in the epididymis of the tammar wallaby (Macropus eugenii) with a 45-kDa acrosome-membrane-associated protein https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:1512 Sat 24 Mar 2018 08:30:26 AEDT ]]> Post-testicular sperm maturation and identification of an epididymal protein in the Japanese quail (<i>Coturnix coturnix japonica</i>) https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:21362 Sat 24 Mar 2018 07:51:24 AEDT ]]> Identification of cytochrome-b5 reductase as the enzyme responsible for NADH-dependent lucigenin chemiluminescence in human spermatozoa https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:41 Sat 24 Mar 2018 07:42:12 AEDT ]]> Formation and dissociation of sperm bundles in monotremes https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:27768 Sat 24 Mar 2018 07:40:48 AEDT ]]> Glycogen synthase kinase 3 regulates acrosomal exocytosis in mouse spermatozoa via dynamin phosphorylation https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:27976 50 = 6.7 nM) led to a significant reduction in dynamin phosphorylation (10.3% vs. 27.3%; P < 0.001), acrosomal exocytosis (9.7% vs. 25.7%; P < 0.01), and in vitro fertilization (53% vs. 100%; P < 0.01). GSK3 was shown to be present in developing germ cells where it colocalized with dynamin in the peri-acrosomal domain. However, additional GSK3 was acquired by maturing mouse spermatozoa within the male reproductive tract, via a novel mechanism involving direct interaction of sperm heads with extracellular structures known as epididymal dense bodies. These data reveal a novel mode for the cellular acquisition of a protein kinase and identify a key role for GSK3 in the regulation of sperm maturation and acrosomal exocytosis.—Reid, A. T., Anderson, A. L., Roman, S. D., McLaughlin, E. A., McCluskey, A., Robinson, P. J., Aitken, R. J., Nixon, B. Glycogen synthase kinase 3 regulates acrosomal exocytosis in mouse spermatozoa via dynamin phosphorylation.]]> Sat 24 Mar 2018 07:38:43 AEDT ]]> The microRNA signature of mouse spermatozoa is substantially modified during epididymal maturation https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:26158 Sat 24 Mar 2018 07:35:27 AEDT ]]> Analysis of protein thiol changes occurring during rat sperm epididymal maturation https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:27256 Sat 24 Mar 2018 07:29:12 AEDT ]]> Proteomic insights into the maturation and capacitation of mammalian spermatozoa https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:22257 Sat 24 Mar 2018 07:17:39 AEDT ]]> Functional characterization of dynamin in spermatozoa epididymal maturation and acrosomal exocytosis https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:34873 Mon 23 Sep 2019 11:30:18 AEST ]]> A novel role for milk fat globule-EGF factor 8 protein (MFGE8) in the mediation of mouse sperm–extracellular vesicle interactions https://novaprd-lb.newcastle.edu.au/vital/access/manager/Repository/uon:49349 Fri 12 May 2023 12:00:00 AEST ]]>