- Title
- Roles for T/B lymphocytes and ILC2s in experimental chronic obstructive pulmonary disease
- Creator
- Donovan, Chantal; Starkey, Malcolm R.; Horvat, Jay C.; Wark, Peter A.; Foster, Paul S.; McKenzie, Andrew N. J.; Hansbro, Philip M.; Kim, Richard Y.; Rana, Batika M. J.; Barlow, Jillian L.; Jones, Bernadette; Haw, Tatt Jhong; Mono Nair, Prema; Budden, Kurtis; Cameron, Guy J. M.
- Relation
- NHMRC
- Relation
- Journal of Leukocyte Biology Vol. 105, Issue 1, p. 143-150
- Publisher Link
- http://dx.doi.org/10.1002/JLB.3AB0518-178R
- Publisher
- John Wiley & Sons
- Resource Type
- journal article
- Date
- 2019
- Description
- Pulmonary inflammation in chronic obstructive pulmonary disease (COPD) is characterized by both innate and adaptive immune responses; however, their specific roles in the pathogenesis of COPD are unclear. Therefore, we investigated the roles of T and B lymphocytes and group 2 innate lymphoid cells (ILC2s) in airway inflammation and remodelling, and lung function in an experimental model of COPD using mice that specifically lack these cells (Rag1 -/- and Rora fl/fl Il7r Cre [ILC2-deficient] mice). Wild-type (WT) C57BL/6 mice, Rag1 -/- , and Rora fl/fl Il7r Cre mice were exposed to cigarette smoke (CS; 12 cigarettes twice a day, 5 days a week) for up to 12 weeks, and airway inflammation, airway remodelling (collagen deposition and alveolar enlargement), and lung function were assessed. WT, Rag1 -/- , and ILC2-deficient mice exposed to CS had similar levels of airway inflammation and impaired lung function. CS exposure increased small airway collagen deposition in WT mice. Rag1 -/- normal air- and CS-exposed mice had significantly increased collagen deposition compared to similarly exposed WT mice, which was associated with increases in IL-33, IL-13, and ILC2 numbers. CS-exposed Rora fl/fl Il7r Cre mice were protected from emphysema, but had increased IL-33/IL-13 expression and collagen deposition compared to WT CS-exposed mice. T/B lymphocytes and ILC2s play roles in airway collagen deposition/fibrosis, but not inflammation, in experimental COPD.
- Subject
- COPD; emphysema; ILC2s; inflammation; remodelling; T cells
- Identifier
- http://hdl.handle.net/1959.13/1424242
- Identifier
- uon:38039
- Identifier
- ISSN:0741-5400
- Rights
- © The Author(s) 2018. Society for Leukocyte Biology Published by Wiley Periodicals, Inc. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
- Language
- eng
- Full Text
- Reviewed
- Hits: 7097
- Visitors: 7395
- Downloads: 328
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | ATTACHMENT02 | Publisher version (open access) | 1 MB | Adobe Acrobat PDF | View Details Download |