Unique protein kinase C profile in mouse oocytes: lack of calcium-dependent conventional isoforms suggested by rtPCR and Western blotting

Title: Unique protein kinase C profile in mouse oocytes: lack of calcium-dependent conventional isoforms suggested by rtPCR and Western blotting
Creator: Gangeswaran, Rathi; Jones, Keith T.
Relation: FEBS Letters Vol. 412, Issue 2, p. 309-312
Publisher Link: http://dx.doi.org/10.1016/S0014-5793(97)00782-5
Publisher: Elsevier BV
Resource Type: journal article
Date: 1997
Description: rtPCR and Western blotting were used to determine which members of the PKC family are present in both immature and mature mouse oocytes. Using isoform-specific PCR primers and antibodies PKC-δ and -λ were detected while such techniques failed to observe the conventional isoforms of PKC-α, -β, -γ. This isoform profile was confirmed using an alternative PCR strategy, which allowed discrimination of PCR products derived from conventional and novel PKC isoforms. In addition PKC-ε, -η, -θ and -ζ were not detected by rtPCR. These results suggest that the predominant isoforms in oocytes are PKC-δ and -λ.
Subject: protein kinase C; oocytes; calcium; fertilization; mouse; PCR
Identifier: uon:6586
Identifier: http://hdl.handle.net/1959.13/804311
Identifier: ISSN:0014-5793
Language: eng
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